Isolation and functional characterization of the pheromone biosynthesis activating neuropeptide receptor of Chinese oak silkworm, Antheraea pernyi
Author：Li Jiang, Fang Zhang, Yang Hou, Kiran Thakur, Fei Hu, Jian-Guo Zhang, Xing-Fu Jiang, Yan-Qun Liu, Zhao-Jun Wei 《International Journal of Biological Macromolecules》
ABSTRACTS
Insect pheromone biosynthesis activating neuropeptide (PBAN) controls the synthesis and actuating of sex pheromones of female adult. In the current examination, the full-length cDNA encoding the PBAN receptor was cloned from the pheromone gland (PG) of Antheraea pernyi (AntpePBANR). The AntpePBANR displayed the characteristic seven transmembrane areas of the G protein-coupled receptor (GPCR) and was closely related to the PBANR from Bombyx mori and Manduca sexta in the phylogenetic tree. The AntpePBANR expressed in mammalian cell lines were enacted by AntpePBAN in a concentration-dependent manner. AntpePBANR activation resulted in the calcium mobilization but did not activate the cAMP elevation pathway. Cells expressing AntpePBANR were profoundly responsive to Antpe-γ-SGNP (suboesophageal ganglion neuropeptides) and Antpe-DH (diapause hormone), different individuals from FXPRLamide (X?=?T, S or V) family in A. pernyi. Deletion of residues in the C-terminal hexapeptide (FSPRLamide) proved that P, R and L played the key parts in initiating the AntpePBANR, the amination to the last C terminal residues which can also likewise impact the activation of AntpePBAN receptor altogether. The mRNA of the AntpePBANR gene demonstrated the most noteworthy transcript levels in pheromone gland followed by fat body.
KEY WORDS
Pheromone biosynthesis activating neuropeptide; G protein-coupled receptors
SCREENSHOT
RELATED PRODUCTS
AntpePBAN, AntpeDH, Antpeα-SGNP, Antpeβ-SGNP, Antpeγ-SGNP and other mimetic peptides were synthesized by ChinaPeptides Co., Ltd. (Shanghai).
CHAINING
https://www.sciencedirect.com/science/article/pii/S0141813018318373
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